Cytochemical Demonstration of Ketosteroids

On the cytochemical demonstration of aldehydes.

IN studying the distribution in tissues of substances which react with reduced fuchsin, it is frequently necessary to distinguish between (a) substances which react as though they were free aldehydes; (A) substances which react as though they were aldehyde acetals; and (c) substances which react as aldehydes as a result of oxidation of precursors in the tissue. In a recent paper on the plasmal ...

Cytochemical demonstration of hydrogen peroxide in polymorphonuclear leukocyte phagosomes

Phagocytosis by polymorphonuclear leukocytes (PMN) is accompanied by specific morphological and metabolic events which may result in the killing of internalized micro-organism. Hydrogen peroxide is produced in increased amounts during phagocytosis (17) and in combination with myeloperoxidase and halide ions constitute a potent, microbicidal mechanism (8,9,11). There can be direct iodination of ...

Rapid cytochemical demonstration of cytochrome oxidase activity in pathogenic bacteria.

AIMS To develop and assess a cytochemical technique for the light microscopical detection of oxidase activity in pathogenic bacteria. METHODS Live bacterial cells were deposited on to aminopropylsilane treated glass coverslips by centrifugation, dried, then reacted with either 1% (w:v) n,n,n',n'-tetramethyl-p-phenylene diamine (TPD) or 5 mM diaminobenzidine (DAB) at 37 degrees C. The preparat...

Formalin Fixation in the Cytochemical Demonstration of Succinic Dehydrogenase of Mitochondria

A variety of established methods for protecting mitochondria were tested on rat duodenal epithelium during the histochemical assay for succinic dehydrogenase. The use of sucrose at isotonic or hypertonic concentrations, 7.5 per cent polyvinylpyrrolidone, divalent cations, physiological salt solutions, phenazine methosulfate, coenzyme Q(10), and menadione failed to improve the quality of the his...

Cytochemical Demonstration of a Single Peroxisome in a Filamentous Green Alga